BOSHY Fluorescent Probe Technology Platform
Next-Generation Lipid Droplet Imaging Solution:
Multi-color Compatible / High Aggregation Emission / High S/N
Lipid droplets are central organelles for cellular energy metabolism, and their dynamics are closely related to various metabolic diseases. Addressing issues like ACQ in traditional BODIPY 493/503 probes, OmiChem introduces the "Two-step One-pot" synthesized BOSHY (Boron Salicylhydrazone) series probes. This platform features large Stokes shift, high aggregation-induced emission (AIE-active), and high lipid droplet specificity, providing a complete technical guide from molecular characteristics to experimental protocols.
Core Innovation & Molecular Design
Unique Non-Planar Molecular Structure, Breaking Traditional Limitations
Twisted Tetrahedral Boron Center
Breaks molecular planarity, effectively inhibiting π-π stacking, enabling solid-state luminescence.
6,7-Fused Bicyclic Rigid Skeleton
Reduces non-radiative transition vibration, significantly improving quantum yield.
O-B-N Coordination Bond
Forms a stable four-coordinate chelate ring, endowing the molecule with excellent chemical stability.
OmiFluo™ BOSHY-LD Yellow vs Green Technical Specifications
| Parameter | OmiFluo™ BOSHY-LD Yellow | OmiFluo™ BOSHY-LD Green | Test Conditions / Notes |
|---|---|---|---|
| Chemical Name | Julolidine-based BOSHY derivative | Methoxy-substituted BOSHY analog | - |
| Molecular Weight (MW) | ~584.26 | 470.25 | - |
| Molecular Properties | LogP 8.12 (High Lipophilicity) | LogP approx. 6-7 | LogP higher than common dyes |
| Absorption Max (λₐbₛ) | 471 nm | 468 nm | Dichloromethane solution |
| Molar Extinction Coeff (ε) | 4.01×10⁴ M⁻¹cm⁻¹ | 4.31×10⁴ M⁻¹cm⁻¹ | Dichloromethane solution |
| Emission Max (λₑₘ) | 586 nm (Soln) / 607 nm (Solid) | 572 nm (Soln) / 590 nm (Solid) | Solvent-dependent features |
| Stokes Shift | ~115 nm / ~7200 cm⁻¹ | ~104 nm / ~6800 cm⁻¹ | Avoids self-absorption |
| Quantum Yield (Φ_F) | 63% (Soln) / 31% (Solid) | 74% (Soln) / 52% (Solid) | Overcomes ACQ effect |
| Fluorescence Lifetime (τ) | 2.69 ns | 2.79 ns | In solution |
| Lipid Droplet Specificity | Pearson Coeff: 0.96-0.98 | Pearson Coeff: 0.93 | HeLa cell validation |
| Cytotoxicity | >90% Viability (50 μM, 24h) | >90% Viability (50 μM, 24h) | CCK-8 assay |
| Purchase Link | Buy Now | Buy Now | - |
Molecular Structure of OmiFluo™ BOSHY-LD Yellow and Green
BOSHY vs Traditional Probes: Comprehensive Analysis
BOSHY Unique Advantages
- Ultra-large Stokes Shift (>100nm): Complete separation of excitation and emission, near-zero background interference.
- High Efficiency Dual-State Emission: Solution Φ_F up to 74%, overcomes ACQ, remains bright in aggregated state.
- Tunable Structure Platform: Emission spectrum 500-620nm continuously tunable, easy for derivatization.
- Chiral Optical Properties: Potential for CPL (Circularly Polarized Luminescence) (g_lum up to 1.7×10⁻⁴).
Technical Points to Note
- Molar Extinction Coefficient: Slightly lower than BODIPY (~4×10⁴), recommend using slightly stronger excitation.
- High Lipophilicity: Concentration and incubation time optimization required to prevent excessive retention.
- Photostability: Better than fluorescein, but long-term photobleaching characteristics need improvement (vs BODIPY).
Why is BODIPY 493/503 Still Irreplaceable?
Core Status of BODIPY 493/503
Although BOSHY has excellent performance, BODIPY 493/503 remains the industry "Gold Standard":
- Technical Maturity: 20+ years of application history, standardized data analysis models.
- Balanced Lipophilicity: Moderate LogP (4-5) ensures fast staining equilibrium and membrane permeability.
- Excellent Photostability: >90% fluorescence retention after 5 min continuous irradiation, preferred for long-term live cell imaging.
Experimental Protocol
Stock Solution Preparation
Formulation: 1.0 mg OmiFluo™ BOSHY-LD Yellow (MW≈670) + 298.5 μL anhydrous DMSO.
Storage: 5 mM stock solution, store at -20°C protected from light.
* Working solution must be prepared fresh.
Cell Staining
Working Solution: 10 μM (Stock diluted 1:500 in serum-free medium).
Incubation: 1 μM final concentration, incubate at 37°C for 20 minutes.
Washing: Wash twice with PBS, replace with complete medium.
Confocal Imaging
Excitation: 488 nm (Argon laser).
Emission Collection: 520-620 nm (for OmiFluo™ BOSHY-LD Yellow).
Objective: 63× Oil Objective (NA=1.4).
Application Guide
Wide Applicability from Basic Research to High Throughput Screening
Fatty Liver Degeneration Model
Main Challenge: Validating correlation between new probes and traditional data.
Recommended Solution: BODIPY 493/503 + OmiFluo™ BOSHY-LD Yellow Dual Labeling. utilizes BOSHY's large Stokes shift to avoid color crosstalk while providing a gold standard reference.
Lipid Droplet-Mitochondria Contact (Mito-LD)
Main Challenge: Avoiding excitation crosstalk during dynamic processes.
Recommended Solution: OmiFluo™ BOSHY-LD Green (572nm) + MitoTracker Deep Red (670nm). Dual-color simultaneous excitation, real-time observation of energy stress dynamics.
Metabolic Drug Screening Platform
Main Challenge: Cost control and data stability.
Recommended Solution: 384-well plate + OmiFluo™ BOSHY-LD Yellow. Cost as low as $0.10/well, high S/N ratio, screening >10,000 compounds/day.