Organelle detection is a fundamental aspect of cell biology research, enabling the identification, localization, and functional analysis of specific intracellular structures such as mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes, and peroxisomes. Detection techniques typically employ fluorescent probes, antibody labeling, or genetically encoded fluorescent proteins to achieve specific staining and imaging. These probes can sense parameters such as membrane potential, pH, redox state, or ion concentration, providing insights into cellular metabolism, stress responses, and apoptosis. Widely used in live-cell imaging, drug screening, toxicology, and disease mechanism studies, organelle detection supports confocal microscopy, super-resolution imaging, and flow cytometric analysis.